Mannosylated Clodrosome Macrophage Depletion Kits

  • Contain unmodified multilamellar liposomes similar to those used in most published depletion studies
  • 4-Aminophenyl-alpha-D-Mannopyranoside incorporated into the liposomes
  • Liposome size range (0.3-3 µm) is optimal for immediate phagocyte recognition and uptake
  • Kits contain equal volumes of each reagent packaged in stoppered glass vials with multidose vial adapters
  • Select the Fluorescent Kit to visualize control liposomes in vitro or in vivo

Each Clodrosome Macrophage Depletion Kit contains Clodrosome and an equal volume of control liposomes.

Further details on each reagent is available by clicking on “More Info” below its image.


m-Clodrosome® (Liposomal Clodronate)

Mannosylated Clodronate liposomes

Mannosylated Clodronate liposomes

More Info
mg/ml mM
Clodronate Disodium Salt 5 17
L-α-Phosphatidylcholine 18.8  24
Cholesterol  4.2  11
4-Aminophenyl-alpha-D-Mannopyranoside  1  3.69
Suspended in Phosphate Buffered Saline at pH 7.4
  • Clodronate encapsulated inside multilamellar liposome aqueous spaces
  • Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
  • Prepared and packaged under sterile conditions

Mannosylayed control liposomes complete the mannosylated Clodrosome Macrophage Depletion Kit.

Select either m-Encapsome control liposomes (Mannosylated Clodrosome Kit) or

m-Fluoroliposome fluorescent control liposomes (Mannosylated Fluorescent Clodrosome Kit) described below.

See FAQ (Question 1) for more information on the use of fluorescent liposomes.


m-Encapsome® (Control Liposomes)

Mannosylated control liposomes

Mannosylated control liposomes

More Info
mg/ml mM
L-α-Phosphatidylcholine 18.8 24
Cholesterol 4.2 11
4-Aminophenyl-alpha-D-Mannopyranoside  1  3.69
Suspended in Phosphate Buffered Saline at pH 7.4
  • Prepared identically to Clodrosome except that clodronate is not added
  • Required control since phagocytes can respond to liposome uptake
  • Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
  • Prepared and packaged under sterile conditions

m-Fluoroliposome®-DiI (Mannosylated Fluorescent Control Liposomes)

Mannosylated fluorescent liposomes w/ DiI

Mannosylated fluorescent liposomes w/ DiI

More Info
mg/ml mM
L-α-Phosphatidylcholine 18.8 24
Cholesterol 4.2 11
4-Aminophenyl-alpha-D-Mannopyranoside  1  3.69
DiI (lipophilic fluorescent dye: ex/em~550/580 nm) 0.0625 0.065
Suspended in Phosphate Buffered Saline at pH 7.4
  • Prepared identically to Clodrosome (without clodronate; with fluorophore)
  • Required control since phagocytes can respond to liposome uptake
  • May be tracked by fluorescence methods (confocal microscopy, FACS, etc.)
  • Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
  • Prepared and packaged under sterile conditions

m-Fluoroliposome®-DiO (Mannosylated Fluorescent Control Liposomes)

Mannosylated fluorescent liposomes w/DiO

Mannosylated fluorescent liposomes w/DiO

More Info
mg/ml mM
L-α-Phosphatidylcholine 18.8 24
Cholesterol 4.2 11
4-Aminophenyl-alpha-D-Mannopyranoside  1  3.69
DiO (lipophilic fluorescent dye: ex/em~484/501 nm) 0.0625 0.071
Suspended in Phosphate Buffered Saline at pH 7.4
  • Prepared identically to Clodrosome (without clodronate; with fluorophore)
  • Required control since phagocytes can respond to liposome uptake
  • May be tracked by fluorescence methods (confocal microscopy, FACS, etc.)
  • Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
  • Prepared and packaged under sterile conditions

m-Fluoroliposome®-DiD (Mannosylated Fluorescent Control Liposomes)

Mannosylated fluorescent liposomes w/DiD

Mannosylated fluorescent liposomes w/DiD

 

More Info
mg/ml mM
L-α-Phosphatidylcholine 18.8 24
Cholesterol 4.2 11
4-Aminophenyl-alpha-D-Mannopyranoside  1  3.69
DiD (lipophilic fluorescent dye: ex/em~648/680 nm) 0.00625 0.0065
Suspended in Phosphate Buffered Saline at pH 7.4
  • Prepared identically to Clodrosome (without clodronate; with fluorophore)
  • Required control since phagocytes can respond to liposome uptake
  • May be tracked by fluorescence methods (confocal microscopy, FACS, etc.)
  • Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
  • Prepared and packaged under sterile conditions

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