Used for…

  • FACS analysis of cells with bound and/or phagocytosed liposomes
  • Liposome trafficking using confocal microscopy in vitro and in vivo
  • Tissue distribution of liposomes administered in vivo
  • Circulation time of liposomes in bloodstream

 

Mannosylated fluorescent liposomes w/ DiI

Mannosylated fluorescent liposomes w/ DiI

Contains: mg/ml mM
L-α-Phosphatidylcholine 18.8 24
Cholesterol 4.2 11
4-Aminophenyl-alpha-D-Mannopyranoside 1 3.69
DiI (lipophilic fluorescent dye: ex/em~550/580 nm) 0.0625 0.065
Suspended in Phosphate Buffered Saline at pH 7.4

 

 

DiI Fluorescence Spectra

DiI Structure


» Prepared identically to Clodrosome except no clodronate is added and fluorophore is incorporated
» Serves as Clodrosome control because phagocytes can respond immunologically to liposome uptake
» Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
» Prepared and packaged under sterile conditions
» Some DiI (pronounced “dye-eye”) advantages…

  • Incorporates into the liposome bilayer, therefore liposome remains fluorescent until bilayer structure is completely dissembled even when liposome contents are released
  • Does not readily exchange out of liposomes into cell membranes or other lipid-containing structures (HDL, etc.)
  • Detectable by, and functions in, all fluorescence methods (fluoroscopy, fluorescence resonance energy transfer (FRET), etc.) using a rhodamine filter
  • Minimal  fluorescence in aqueous phase, but high yield when incorporated into lipid bilayers
  • DiI liposomes are highly visible to the naked eye even in small quantities
  • Comprehensive description of DiI from the Molecular Probes Handbook at invitrogen.com
  • See FAQ (Question 1) for more information on the use of fluorescent liposomes.

 

 


» Surface-modified with branched-chain oligomannose for optimal recognition by mannose-receptors (MR, SIGNR1)
» Lipid composition is biosimilar egg-phosphatidylcholine and cholesterol in a 7:3 molar ratio
» Optimal size range (0.3-3 µm) for immediate phagocyte recognition and phagocytosis
» Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
» Prepared and packaged under sterile conditions in 5 ml stoppered glass vials with multidose adapters
» Batches prepared weekly to ensure maximal shelf-life after receipt by client
» Available as single product or in convenient Mannosylated Macrophage Depletion Kits


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