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Fluoroliposome-DiI

Used for…
  • FACS analysis of cells with bound and/or phagocytosed liposomes
  • Liposome trafficking using confocal microscopy in vitro and in vivo
  • Tissue distribution of liposomes administered in vivo
  • Circulation time of liposomes in bloodstream
  Fluoroliposome Injection
Contains: mg/ml mM
L-α-Phosphatidylcholine 18.8 24
Cholesterol 4.2 11
DiI (lipophilic fluorescent dye: ex/em~550/580 nm) 0.0625 0.067
Suspended in Phosphate Buffered Saline at pH 7.4

 

DiI Fluorescence Spectra

DiI Structure


» Prepared identically to Clodrosome except no clodronate is added and fluorophore is incorporated
» Serves as Clodrosome control because phagocytes can respond immunologically to liposome uptake
» Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries
» Prepared and packaged under sterile conditions
» Some DiI (pronounced “dye-eye”) advantages…

  • Incorporates into the liposome bilayer, therefore liposome remains fluorescent until bilayer structure is completely dissembled even when liposome contents are released
  • Does not readily exchange out of liposomes into cell membranes or other lipid-containing structures (HDL, etc.)
  • Detectable by, and functions in, all fluorescence methods (fluoroscopy, fluorescence resonance energy transfer (FRET), etc.) using a rhodamine filter
  • Minimal  fluorescence in aqueous phase, but high yield when incorporated into lipid bilayers
  • DiI liposomes are highly visible to the naked eye even in small quantities
  • Comprehensive description of DiI from the Molecular Probes Handbook at invitrogen.com
  • See FAQ (Question 1) for more information on the use of fluorescent liposomes.

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Contact

Encapsula NanoSciences LLC
6 Cadillac Dr
Suite 245
Brentwood, TN 37027
Phone: 1-800-387-0620
Phone: 1-800-401-5140
Fax: 615-250-8747
URL: www.encapsula.com
email: info@encapsula.com

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Clodrosome®, Encapsome®, and Fluoroliposome® are trademarks of Encapsula NanoSciences. The content of the website can only be used by researchers, educators and students for educational purposes. Any commercial use of the content of the website is legally prohibited by the laws of the United States of America.

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